Monday, 19 September 2016

Human Hair as a Long Term Biological Indicator for Assessing Strontium Nutritional Status


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Strontium (Sr) is a bone seeking trace element that closely parallels calcium metabolism. At the present, there is no available long-term biological indicator for assessing the human Sr nutritional status. In this article, we have presented a novel concept on how to assess the human strontium nutritional status by studying its frequency distribution in the hair and whole blood. In this prospective, observational, cross-sectional, and exploratory epidemiological study, we analyzed hair Sr (SrH) in 311 apparently healthy adult Croatians (123 men, 188 women); among them, whole blood strontium (SrWB) was detected in 142 women and 89 men. Hair and WB samples were analyzed for strontium by the inductively coupled mass plasma spectrometry (ICP MS). The natural frequency distribution of hair strontium obeyed the power law so that the data were analyzed by fitting the logistic bioassay sigmoid curve (ogive) of the median derivatives. Women have accumulated more SrH than men (median men 0.867 µg·g-1 vs. median women 3.120 µg·g-1). The normal physiological response range covered by the linear part of the sigmoid curve was 0.418 - 2.950 µg·g-1 for men and 0.846 - 7.260 µg·g-1 for women. Values below that linear range were considered to denote low Sr nutritional status, whereas values above that range would indicate excessive dietary strontium exposure. The long term biological indicator of hair strontium (SrH) is incommensurable with that of the short term biological indicator of the whole blood strontium (SrWB). On the average, women have about three times more strontium in their hair than men, regardless of the age. Analysis of the hair strontium frequency distribution is a valuable long term biological indicator in assessing the population strontium nutritional status.

Strontium is an osteotrophic (bone-seeking) trace element that is normally present in our diet and our bodies. The pioneers of trace element analysis considered strontium to be an essential trace element for humans , but the conclusive evidence on strontium essentiality for humans has yet to be provided . Strontium was in the limelight in the 50’s and 60’s of the last century when it was discovered that the metabolism of strontium was closely related to the metabolism of calcium in bone tissue. Indeed, 99% of typical body burden of 320 mg Sr is found in the bone. The atmospheric fallout of fission products after nuclear testing included a long-living bone seeker of 90Sr which has a physical half-life of ~ 27 y anda biological T/2 ~ 10 y. Radioactive 90Sr is a powerful beta radiation source and, when lodged in the bones, would act as a strong radio-toxic carcinogen. Moreover, strontium, like calcium, could be transferred from mother to fetus/newborn via the mother’s milk and get deposed in their mineralized tissue with possibly deleterious consequences. Later on, the interest in strontium was renewed when Skoryna demonstrated the pharmacological effect of both the stable and 89Sr radiopharmaceutical isotope Metasteron® to alleviate the pain of mammary and prostate carcinoma bone metastases. Today, the most recent research has shown that strontium ranelate [Protelos®, Osseor®] acts as an effective nutritive agent, capable of slowing down mineral bone loss due to old age. Indeed, these strontium compounds are preventing and delaying the onset and progression of osteoporosis, including both femur and vertebral osteoporosis. How much such a treatment can affect the strontium body burden and the metabolism of other trace elements in the skeleton remains to be elucidated. Since strontium is not as yet recognized to be an essential trace element, there are no recommended dietary allowances (RDA’s) for strontium, and there is also no available long term biological indicator tissue for assessing the strontium nutritional status of the body; the US Environmental Protection Agency has derived an oral recommended dose for strontium of 0.6 mg per kg body weight per day .

Today, hair trace element analysis is accepted as a long-term biological indicator for assessing trace element nutritional status and the level of their environmental exposure. The aim of this study was to demonstrate how strontium hair analysis (SrH) may serve as a long term biological indicator tissue for assessing the strontium nutritional status of the human body by analyzing hair strontium frequency distribution properties with a median derivative method. Thus far, we have shown that our innovative approach yielded a reliable assessment of the nutritional status of the essential trace elements iodine and selenium, and in evaluating the exposure to the non-essential elements of silver and aluminium. The observed data on hair strontium analysis were compared with that for whole blood strontium (SrWB), i.e., a common short term biological indicator tissue for the assessment of numerous bioelements and other different clinical parameters.

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